Legionella Testing Services

Legionella Testing That Tells You What’s Really Happening in Your Water System 

If you don’t test, you’re guessing. Legionella risk can rise quietly in low-flow areas, warm zones, storage, and aerosol-producing equipment—until you’re reacting under pressure. Routine testing helps you validate whether your Water Management Plan is actually controlling risk and gives you a clear signal when corrective action is needed.

The Real Issue: You Can’t Correct What You Can’t Confirm

Many facilities have “maintenance,” but not a clear, measurable view of Legionella conditions across the system.

What You Don't Know

You can’t confirm whether control measures are working 

You don’t know if risk is isolated or widespread 

You’re forced into reactive decisions when a concern arises 

Bottom line: Testing turns uncertainty into an action plan. 

Testing = Validation + Smarter Decisions

ASHRAE Standard 188-2021 emphasizes Water Management Plans (WMPs) and includes a confirmation step—procedures to verify the plan’s effectiveness, often referred to as validation. Routine monitoring supports that validation and highlights where reassessment may be needed. 

Legionella Water Testing Services

We coordinate Legionella testing through certified microbiology laboratory partners and help you choose the right method, sampling plan, and cadence for your building and risk profile.

Culture Testing (Gold Standard)

The gold standard for Legionella detection. Ideal for routine validation and detailed investigations, with optional serogrouping.

Rapid Testing (qPCR & Legiolert)

Fast turnaround results—qPCR in hours, Legiolert in ~7 days—helping you make quicker decisions while maintaining accuracy.

Swab Testing

Useful during targeted investigations or hard-to-reach locations. Helps identify issues inside fixtures, dead legs, and problem points.

Test Strips & Monitoring Tools

On-site test strips, handheld meters, and monitoring tools that support routine checks on disinfectant, temperature, and system performance.

Testing Frequency (How Often Should You Sample?)

There isn’t one universal answer. Frequency should be based on a risk assessment and a sampling plan that represents each water system. 

Common starting points include: 

  • Potable hot water: quarterly testing with enough locations to represent near/mid/far points (and key outlets like ice machines) 
  • Cooling towers: monthly basin testing while in operation 
  • Decorative fountains: monthly in warm months, less frequent in cooler months 

We’ll help you build a practical plan that fits your building’s realities (usage patterns, maintenance practices, and any prior history). 

How to Collect Samples

Sampling technique matters—bad sampling can create misleading results. 

How it Works

Sample The Problem

Don’t flush before sampling. A first-draw sample reflects real conditions inside the plumbing.

Always use bottles containing sodium thiosulfate. It neutralizes disinfectants like chlorine so results remain accurate.

Record date, time, and sample location. Good documentation is essential for interpreting results.

Collect from the sump, away from chemical injection points, and not immediately after a normal biocide event.

If You Get a Positive Result

The most important thing: don’t improvise—follow the plan 

A positive result is serious, but the next steps should be structured. Your Water Management Plan (WMP) should define exactly what to do when results indicate poorly controlled or uncontrolled growth.  

For high-risk settings, interim protective measures (like point-of-use filtration) may be considered while corrective actions are executed. 

The CDC recommends the following actions for poorly controlled or uncontrolled Legionella growth: 

  • Review sample collection, handling, and testing procedures for potential errors. 
  • Verify that water system equipment is functioning correctly and operating as intended. 
  • Conduct a records review to confirm that the WMP is being implemented properly. 
  • Verify operating conditions, such as the physical and chemical characteristics of incoming water and facility water systems. 
  • Re-evaluate aspects of the WMP, including risk assessment, cleaning, maintenance procedures, chemical treatment, and other factors affecting Legionella testing. 
  • Correct deficiencies by adjusting the WMP as necessary. 
  • Consider whether remediation is required after completing the first six steps. 
  • If remediation is performed, wait at least 48 hours after the system returns to normal before retesting for Legionella. 
Legionella in Cooling Tower Water Solutions

The CDC Published Guidance in 2021 Regarding Positive Legionella Test Results.

Historically, the Centers for Disease Control and Prevention (CDC) had no official stance on the results of Legionella testing. 2021 marked the first year they published guidance for the public. The CDC’s graphic “Figure 1. Routine Legionella testing: A multifactorial approach to performance indicator interpretation” highlights four areas of consideration for Legionella testing results. The first section discusses which concentration indicates that Legionella growth appears uncontrolled, poorly controlled, or well controlled. Concentrations expressed as CFU/mL are for test results by traditional plate culture methods. If other test methods are used, consult an expert for interpretation. 

The detection of greater than or equal to 10 CFU/mL in potable water or the detection of greater than or equal to 100 CFU/mL in non-potable water indicates that Legionella growth appears uncontrolled. 

The detection of 1.0-9.9 CFU/mL in potable water or the detection of 10-99 CFU/mL in non-potable water indicates that Legionella growth appears poorly controlled. 

The detection of any to 0.9 CFU/mL in potable water or the detection of any to 9 CFU/mL in non-potable water indicates that Legionella growth appears well controlled. 

No Legionella detected also indicates that Legionella growth appears well controlled, and is a stronger indication that Legionella is well controlled than detection of any Legionella. 

The second section relates to the way in which concentration change over time indicates that Legionella growth appears uncontrolled, poorly controlled, or well controlled. 

A 100-fold or greater increase in concentration (for example, an increase from 0.7 CFU/mL to 70 CFU/mL) indicates that Legionella growth appears uncontrolled. 

A 10-fold increase in concentration (for example, an increase from 0.7 CFU/mL to 7 CFU/mL) indicates that Legionella growth appears poorly controlled. 

Steady Legionella concentration (for example, 0.7 CFU/mL for two consecutive sampling rounds) indicates that Legionella growth appears well controlled. 

NSF Certified Chemicals

Our formulations meet NSF safety standards to protect your people and your equipment.

50+ Years of Expertise

Backed by decades of industry experience and a proven track record of results.

Regional Expertise

Serving facilities across the midwest with responsive, local water treatment support.

Stop Guessing. Start Controlling.

ChemREADY helps you select the right test method, build a realistic sampling plan, and translate results into clear next steps—so you’re not stuck with data you can’t act on.

Our Services

Closed Loop Water Treatment

Closed loops feed your heat exchangers too. Explore our Closed Loop Water Treatment and Glycol Solutions to stop corrosion before it reaches your boiler.

Cooling Tower Solutions

Your steam system isn’t the only one that needs protection. Keep your cooling water clean and balanced with our Cooling Tower Treatment Programs.

Filtration Solutions

For maximum reliability, combine your boiler program with Filtration Solutions to remove rust and debris before they enter your feedwater system

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